dna dna relatedness 251 Search Results


dna dna relatedness 251  (ATCC)
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Dna Dna Relatedness 251, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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251 methods 252 sample preparation 253 cell free dna cfdna  (Thermo Fisher)
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Thermo Fisher 251 methods 252 sample preparation 253 cell free dna cfdna
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bst dna polymerase  (OptiGene Ltd)
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drought medicago trunc  (Medicago)
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a commercial dna extraction kit  (Qiagen)
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Qiagen a commercial dna extraction kit
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251 nt dna template  (Danaher Inc)
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rna pol ii sc 899  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology rna pol ii sc 899
5′ RLM-RACE determinations of MKLP1 (A), Ect2 (B), and RacGAP (C) promoter TSS. mRNAs were prepared from S-phase-synchronized Kit 225 cells. Shown to the left are results of 6% acrylamide gel electrophoresis of the RLM-RACE products. At right are schematic alignments of obtained sequences with GenBank references. Light-gray arrows indicate the most 5′ nucleotides. The number of independent clones that were sequenced is shown underneath. (D) Recruitment of <t>RNA</t> <t>Pol</t> <t>II</t> to proximal promoters upon IL-2 stimulation of Kit 225 cells as assessed by ChIP analysis using anti-Pol II or control (Ctrl; anti-HA) antibodies. Primers used for PCR amplification of ChIP products are listed in File S3 in the supplemental material. Input represents 2% of the starting material. IP, immunoprecipitation.
Rna Pol Ii Sc 899, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ezna stool dna kit d4015  (Omega Bio Tek)
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5′ RLM-RACE determinations of MKLP1 (A), Ect2 (B), and RacGAP (C) promoter TSS. mRNAs were prepared from S-phase-synchronized Kit 225 cells. Shown to the left are results of 6% acrylamide gel electrophoresis of the RLM-RACE products. At right are schematic alignments of obtained sequences with GenBank references. Light-gray arrows indicate the most 5′ nucleotides. The number of independent clones that were sequenced is shown underneath. (D) Recruitment of <t>RNA</t> <t>Pol</t> <t>II</t> to proximal promoters upon IL-2 stimulation of Kit 225 cells as assessed by ChIP analysis using anti-Pol II or control (Ctrl; anti-HA) antibodies. Primers used for PCR amplification of ChIP products are listed in File S3 in the supplemental material. Input represents 2% of the starting material. IP, immunoprecipitation.
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superscript vilo cdna synthesis kit  (Thermo Fisher)
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Thermo Fisher superscript vilo cdna synthesis kit
5′ RLM-RACE determinations of MKLP1 (A), Ect2 (B), and RacGAP (C) promoter TSS. mRNAs were prepared from S-phase-synchronized Kit 225 cells. Shown to the left are results of 6% acrylamide gel electrophoresis of the RLM-RACE products. At right are schematic alignments of obtained sequences with GenBank references. Light-gray arrows indicate the most 5′ nucleotides. The number of independent clones that were sequenced is shown underneath. (D) Recruitment of <t>RNA</t> <t>Pol</t> <t>II</t> to proximal promoters upon IL-2 stimulation of Kit 225 cells as assessed by ChIP analysis using anti-Pol II or control (Ctrl; anti-HA) antibodies. Primers used for PCR amplification of ChIP products are listed in File S3 in the supplemental material. Input represents 2% of the starting material. IP, immunoprecipitation.
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high-capacity cdna reverse transcription kit  (Thermo Fisher)
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Thermo Fisher high-capacity cdna reverse transcription kit
5′ RLM-RACE determinations of MKLP1 (A), Ect2 (B), and RacGAP (C) promoter TSS. mRNAs were prepared from S-phase-synchronized Kit 225 cells. Shown to the left are results of 6% acrylamide gel electrophoresis of the RLM-RACE products. At right are schematic alignments of obtained sequences with GenBank references. Light-gray arrows indicate the most 5′ nucleotides. The number of independent clones that were sequenced is shown underneath. (D) Recruitment of <t>RNA</t> <t>Pol</t> <t>II</t> to proximal promoters upon IL-2 stimulation of Kit 225 cells as assessed by ChIP analysis using anti-Pol II or control (Ctrl; anti-HA) antibodies. Primers used for PCR amplification of ChIP products are listed in File S3 in the supplemental material. Input represents 2% of the starting material. IP, immunoprecipitation.
High Capacity Cdna Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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drought medicago truncatula  (Medicago)
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Medicago drought medicago truncatula
5′ RLM-RACE determinations of MKLP1 (A), Ect2 (B), and RacGAP (C) promoter TSS. mRNAs were prepared from S-phase-synchronized Kit 225 cells. Shown to the left are results of 6% acrylamide gel electrophoresis of the RLM-RACE products. At right are schematic alignments of obtained sequences with GenBank references. Light-gray arrows indicate the most 5′ nucleotides. The number of independent clones that were sequenced is shown underneath. (D) Recruitment of <t>RNA</t> <t>Pol</t> <t>II</t> to proximal promoters upon IL-2 stimulation of Kit 225 cells as assessed by ChIP analysis using anti-Pol II or control (Ctrl; anti-HA) antibodies. Primers used for PCR amplification of ChIP products are listed in File S3 in the supplemental material. Input represents 2% of the starting material. IP, immunoprecipitation.
Drought Medicago Truncatula, supplied by Medicago, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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high capacity cdna reverse transcription kit  (Thermo Fisher)
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Thermo Fisher high capacity cdna reverse transcription kit
5′ RLM-RACE determinations of MKLP1 (A), Ect2 (B), and RacGAP (C) promoter TSS. mRNAs were prepared from S-phase-synchronized Kit 225 cells. Shown to the left are results of 6% acrylamide gel electrophoresis of the RLM-RACE products. At right are schematic alignments of obtained sequences with GenBank references. Light-gray arrows indicate the most 5′ nucleotides. The number of independent clones that were sequenced is shown underneath. (D) Recruitment of <t>RNA</t> <t>Pol</t> <t>II</t> to proximal promoters upon IL-2 stimulation of Kit 225 cells as assessed by ChIP analysis using anti-Pol II or control (Ctrl; anti-HA) antibodies. Primers used for PCR amplification of ChIP products are listed in File S3 in the supplemental material. Input represents 2% of the starting material. IP, immunoprecipitation.
High Capacity Cdna Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


5′ RLM-RACE determinations of MKLP1 (A), Ect2 (B), and RacGAP (C) promoter TSS. mRNAs were prepared from S-phase-synchronized Kit 225 cells. Shown to the left are results of 6% acrylamide gel electrophoresis of the RLM-RACE products. At right are schematic alignments of obtained sequences with GenBank references. Light-gray arrows indicate the most 5′ nucleotides. The number of independent clones that were sequenced is shown underneath. (D) Recruitment of RNA Pol II to proximal promoters upon IL-2 stimulation of Kit 225 cells as assessed by ChIP analysis using anti-Pol II or control (Ctrl; anti-HA) antibodies. Primers used for PCR amplification of ChIP products are listed in File S3 in the supplemental material. Input represents 2% of the starting material. IP, immunoprecipitation.

Journal:

Article Title: CUX1 and E2F1 Regulate Coordinated Expression of the Mitotic Complex Genes Ect2 , MgcRacGAP , and MKLP1 in S Phase ▿ †

doi: 10.1128/MCB.01275-08

Figure Lengend Snippet: 5′ RLM-RACE determinations of MKLP1 (A), Ect2 (B), and RacGAP (C) promoter TSS. mRNAs were prepared from S-phase-synchronized Kit 225 cells. Shown to the left are results of 6% acrylamide gel electrophoresis of the RLM-RACE products. At right are schematic alignments of obtained sequences with GenBank references. Light-gray arrows indicate the most 5′ nucleotides. The number of independent clones that were sequenced is shown underneath. (D) Recruitment of RNA Pol II to proximal promoters upon IL-2 stimulation of Kit 225 cells as assessed by ChIP analysis using anti-Pol II or control (Ctrl; anti-HA) antibodies. Primers used for PCR amplification of ChIP products are listed in File S3 in the supplemental material. Input represents 2% of the starting material. IP, immunoprecipitation.

Article Snippet: Antibodies to RNA Pol II (sc-899) and E2F-1 (sc-251) were from Santa Cruz Biotechnology.

Techniques: Acrylamide Gel Assay, Electrophoresis, Clone Assay, Control, Amplification, Immunoprecipitation